A COLORIMETRIC METHOD FOR THE DETERMINATION OF HOMOGENTISIC ACID IN URINE
نویسندگان
چکیده
منابع مشابه
Determination of homogentisic acid in urine.
A relatively simple method for the quantitative determination of homogentisic acid in urine is presented. Homogentisic acid (2,5-dihydroxyphenylacetic acid) is oxidized by atmospheric oxygen in mild alkali to form 1,4-benzoquinone-2-acetic acid. The latter compound is then conjugated with 2,4-dinitrophenylhydrazine. The resulting hydrazone, in the presence of alcoholic sodium hydroxide, produce...
متن کاملAn enzymatic spectrophotometric method for the determination of homogentisic acid in plasma and urine.
Previous methods for the determination of homogentisic acid have been based upon its ability to reduce silver (I), phosphomolybdic acid (a), or iodine (3-5). The error introduced by other biological substances which have similar reducing properties is inconsequential in the determination of the relatively large amounts of homogentisic acid present in urine from a patient with alcaptonuria. Such...
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In the course of feeding experiments in which mono-, di-, and trimethylamines were fed to dogs, it was desirable to determine the urinary excretion of methylamine (monomethylamine). The available methods were found to be cumbersome and not well adapted to the routine analysis of a large number of urines. Weber and Wilson (1) separated ammonia from the amines by the use of yellow mercuric oxide ...
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We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...
متن کاملNew colorimetric method for quantitative determination of protein in urine.
Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1922
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)85887-2